The Molecular Core Facility supports research and education for faculty, students and staff on campus, as well as for off-campus research entities. For a nominal fee, users have unlimited access to technologically advanced equipment for the analysis of DNA, RNA and protein. These resources are well maintained and are secured by a swipe-card system. Learn more about our shared resources.

One-on-one training sessions and personalized experimental services are available for a modest fee. The facility is also used as a teaching space for laboratory courses offered by the Biomedical Sciences Graduate program.

If you have questions about the Molecular Core Facility, its fee structure or the laboratory courses, please contact the Facility Director, Julia A. Sharp, PhD, by email at sharpja@evms.edu.

Services

The DNA sequencing service is provided for a nominal fee of $9 per sample, which covers the reaction, cleanup and electrophoresis. There is a $27 minimum charge. DNA and primers should be supplied in the following concentrations with amounts needed per reaction in parentheses:

  • Plasmids, 75ng/µL (2 µL)
  • PCR products, 10ng/µL (5 µL) 
    • PCR products must be gel purified or otherwise verified to be a single product.
  • Primers, 3.2µM (1 µL); please note the Tm
    • Primers should be 100-150bp upstream of the region of interest.

DNA and primers should be supplied separately and in excess volume. Please note that any sample that is not provided in the appropriate concentration as listed will incur an additional $2 dilution fee for processing.

Results are emailed and provided as a *.ab1 data file.

The microarray service is provided using an Affymetrix Gene Atlas Microarray System. Affymetrix Microarray strips containing four arrays are used to examine Whole Transcriptome (WT) expression or miRNA profiling. A four-sample minimum is required; fees are as follows:

  • $490 per sample for standard WT arrays*
  • $250 - $490 per sample for miRNA arrays*

*Fees may change depending on the specific array required for your experiment; please contact molecularcore@evms.edu for current pricing.

Consultation

Dr. Sharp is available for consultation by appointment. To arrange a meeting, please email us at molecularcore@evms.edu.

Classes and training

Classes and training sessions are scheduled at various times during the year. Please contact us at molecularcore@evms.edu for more information.

Acknowledgments

If the Molecular Core Facility was used for generating data for publication, please acknowledge the facility within your manuscript where appropriate.