The Seahorse Analyzer is housed in the Internal Medicine Department and is available to all researchers through user fees.
The Seahorse (XFe24 Extracellular Flux Analyzer) simultaneously interrogates the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time. The XFe24 Analyzer determines in vitro oxygen consumption rate (OCR), and extracellular acidification rate (ECAR), in order to assess cellular functions such as oxidative phosphorylation, glycolysis, and fatty acid oxidation.
The measurement of cellular bioenergetics on live cells enables time-resolved analysis and testing of multiple conditions per assay well. XF assays provide increased throughput and use less sample compared to conventional respirometry techniques. By incorporating automated compound addition and solid-state fluorescence sensors in a microplate format, XF technology provides the tools to:
- Simultaneously measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in all assay wells.
- Rapidly detect cellular responses to substrates, inhibitors, and other perturbants.
- Test more conditions with the same amount of sample, maximizing the value of each experiment.
The XFe Extracellular Flux Analyzer, XF stress test kits, reagents, consumables, and software tools work together to simplify the measurement of cellular metabolism. The power of this platform has enabled researchers to generate data for over 1000 publications.