Gene Expression Data Evaluation
Gene arrays are used to simultaneously evaluate mRNA levels of a large number of genes in a tissue sample, ranging from as few as ~50 to the transcriptional activity of the entire genome. The data are sample transcript levels and typically compared between test and control groups - in the case of Affymetrix data, we use the Affymetrix® Expression Console™ software evaluation in addition to our own to determine data quality. We evaluate data quality and perform a Core Analysis using the Ingenuity Pathway Analysis (IPA) software that includes a standardized report for most significantly altered transcripts and the underlying upstream regulation present in a system customized fashion. Advanced IPA functions are available by request.