Molecular Core

Molecular Core Facility

 

The Molecular Core supports research and education at EVMS by housing technologically advanced equipment, such as an automated sequencer, real time PCR machine, Odyssey infrared scanner and a Gene Atlas Microarray System for analysis of DNA, RNA and protein.  A detailed listing of the instruments available in the Molecular Core can be accessed by clicking the Shared Resources link.

The Director provides one-on-one training sessions and personalized experimental services for a modest fee. The facility is also used as teaching space for laboratory courses offered by the Biomedical Sciences Graduate Program.

Questions regarding the Molecular Core Facility, its fee structure, and the laboratory courses should be directed to the Facility Director, Dr. Julia A. Sharp. Email: MolecularCore@evms.edu


Services

  • The DNA Sequencing service is provided for a nominal fee of $8 per sequence, with a $24 minimum charge.  DNA should be supplied in the following amounts per reaction: Plasmids at 150ng (75ng/uL), genomic DNA at 300ng (150ng/uL), and/or PCR products at 50ng (10ng/uL).  Primers should be supplied at 3.2pmol/uL, with 1 uL required per reaction.  DNA and primers should be supplied separately and in excess amounts. Results will be provided via a raw data file.

  • The Microarray service is provided using a Gene Atlas Microarray System.  We provide 3’ IVT and Whole Transcript Gene Arrays available from Affymetrix.  The fee is $450 per sample, with a 4-sample minimum.  This fee is based on the pricing for standard arrays and may change depending on the specific array required for your experiment.  This service does not include analysis.

Consultation

Dr. Sharp is available for consultation by appointment. Please email her at MolecularCore@evms.edu to arrange a meeting.


Classes & Training

Classes and Training sessions are scheduled at various times during the year and will be posted here as they are scheduled.


Testimonials


Acknowledgments

If the Molecular Core Facility was utilized for generating data for publication, please acknowledge the facility within your manuscript, where appropriate.