Shared Resources

  • BD FACSCalibur.  An 8-color analysis cytometer, this Cytek-upgraded Calibur now has 3 lasers – blue (488nm), red (637nm), and violet (407nm) – with 8 detectors.  It uses the FlowJo Collectors Edition software for acquisition.
  • Blue/488nm:
    • FL1 (530nm) – FITC
    • FL2 (585nm) – PE
    • FL3 (680nm) – PerCP, or PerCP-Cy5.5
    • FL4 (740nm) – PE-Cy7
  • Red/637nm:
    • FL1 (666nm) – APC
    • FL2 (740nm) – APC-Cy7
  • Violet/407nm
    • FL1 (440nm) – PacBlue
    • FL2 (546nm) – PacOrange
  • Please be aware that the majority of problems with the instrument occur when users do not clean the cytometer properly after use, which leads to clogging the sample delivery line.  This results in machine downtime.  Please be sure to check the tanks before use and follow the posted cleaning and shut-down procedures.
  • Flow Facility staff does not take responsibility for the storage of data; investigators are expected to back up their data from the instrument immediately after use.  The hard drive is cleared once a month of old data.

  • SONY iCyt Reflection.  This high-speed cell sorter and analyzer is equipped with one sorting unit for 2- or 4-way sorting.  It supports BSL2+ sorting and 10 colors of fluorescence using 3 lasers.
  • Blue/488nm:
    • 525nm (FITC), 585nm (PE), 615nm (PE-TR), 700nm (PerCP-Cy5.5), 780nm (PE-Cy7)
  • Red/633nm:
    • 665nm (APC), 720nm (Alexa 700), 780nm (APC-Cy7)
  • Violet/405nm:
    • 455nm (BV421), 585nm (BV570)

     

  • Bio-Plex MAGPIX.  This multiplex reader is a compact, robust system for magnetic bead-based immunoassays, capable of reading assays designed on magnetic xMAP (MagPlex) beads, compatible with Bio-Plex Pro magnetic assays.
    • Read time (1 x 96-well plat): ~60 minutes
    • Plate compatibilities: 96-well only
    • Number of measurable analytes/well: 50
    • Assay compatibility: Magnetic bead-based assays
    • Acquisition software: Bio-Plex Manager MP
    • Analysis software: Bio-Plex Manager software