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FAQ

Pricing FAQ

Q1: I have a 1D gel band from a mouse cell line. How much will it cost to identify the proteins?

A1: For quick analysis (typically identifying between 1 and 10 proteins), we recommend using the fast gradient. For more complex samples, such as gel bands from IP pulldowns or molecular weight fractions of whole cell lysates, we recommend the medium gradient. The largest number of proteins reliably identified in our facility using a medium gradient from a gel band is 73 (approximately 400 peptides).

Fast Gradient:

 

In-gel Digestion

$ 40

LC/MS/MS analysis (gel band - medium gradient)

$ 70

First pass database search (included)

$ 0

Total Cost

$ 110

 

Medium Gradient:

 

In-gel Digestion

$ 40

LC/MS/MS analysis (gel band - medium gradient)

$ 180

First pass database search (included)

$ 0

Total Cost

$ 220

 

Q2: I have two immunoprecipitates, and I want to know what proteins differentiate the samples. How much will this cost?

A2: The researcher is looking for gross differences between these samples, thus this analysis does not require rigorous quantitation to differentiate the samples. A single LC - MSE run will be performed for each sample.

 

Digestion (x2)

$ 80

Gel-free, Label-free Sample Differentiation (test & control)

$ 600

First pass database search (included)

$ 0

Total Cost

$ 680

 

Q3: I am looking for subtle differences in protein expression in a cell line as a function of drug treatment. I am not sure what proteins will be affected. How much will this cost?

A3: To insure accurate quantitation by statistically accounting for analytical variability, it is recommended that each sample be analyzed in triplicate (technical replicates of LC/MS/MS).

 

Bradford Assay and total protein normalization (x2)

$ 80

Digestion (x2)

$ 80

Gel-free, Label-free Sample Differentiation (test & control)

$ 1500

First pass database search (included)

$ 0

AbsQuant or Relative Quant data workup (x2)

$ 300

Total Cost

$ 1960

 

To statistically account for biological and analytical variability, it is recommended that three biological replicates (each test and control) be analyzed, with three technical replicates of each biological replicate. This cost would be:

 

Bradford Assay and total protein normalization (x2)

$ 240

Digestion (x6)

$ 240

Gel-free, Label-free Sample Differentiation (test & control)

$ 4500

First pass database search (included)

$ 0

AbsQuant or Relative Quant data workup (x6)

$ 1800

Total Cost

$ 6780

 

Q4: I want to perform biomarker discovery in human plasma samples. How much will this cost per sample?

A4: It is recommended that 10% of the samples be analyzed in triplicate and 90% of the samples be analyzed in singlicate.

Singlicate Analysis - Single stage of plasma depletion (per sample)

 

Bradford Assay and total protein normalization (x2)

$ 80

Digestion

$ 40

Depletion (Hu14)

$ 300

Gel-free, Label-free Sample Differentiation

$ 300

First pass database search (included)

$ 0

AbsQuant or Relative Quant data workup

$ 150

Total Cost

$ 870

 

Singlicate Analysis - Two stage plasma depletion (per sample)

 

Bradford Assay and total protein normalization (x2)

$ 80

Digestion

$ 40

Depletion (IgY14 + SuperMix))

$ 500

Gel-free, Label-free Sample Differentiation

$ 300

First pass database search (included)

$ 0

AbsQuant or Relative Quant data workup

$ 150

Total Cost

$ 1070

 

Triplicate Analysis - Single stage of plasma depletion (per sample)

 

Bradford Assay and total protein normalization (x2)

$ 80

Digestion

$ 40

Depletion (Hu14)

$ 300

Gel-free, Label-free Sample Differentiation

$ 750

First pass database search (included)

$ 0

AbsQuant or Relative Quant data workup

$ 150

Total Cost

$ 1320

 

Triplicate Analysis - Two stage plasma depletion (per sample)

 

Bradford Assay and total protein normalization (x2)

$ 80

Digestion

$ 40

Depletion (IgY + SuperMix))

$ 500

Gel-free, Label-free Sample Differentiation

$ 300

First pass database search (included)

$ 0

AbsQuant or Relative Quant data workup

$ 150

Total Cost

$ 1520

 

Q5: The species of interest to my study is not in the databases currently mounted. How can I appropriately process my data?

A5: We can upload your custom database to our Mascot server for database searching. This is a one-time fee, after which this database is available for your future use.

 

Custom Database setup for MASCOT

$ 50