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Dianne C. Daniel , PhD

    • Title:
    • Associate Professor

    • Role:
    • Faculty

    • Faculty Appointments:
    • Additional Adjunct Appointment:

      Department of Pathology
      Mount Sinai School of Medicine
      New York, NY

    • Additional Certifications:
    • Focus Areas:
    • Office Location:
    • Lewis Hall

    • Undergraduate Education:
    • Graduate Education:
    • M.S., Longwood College, Farmville, VA

      Ph.D., City University of New York, New York, NY

    • Postdoctoral Education:
    • Mount Sinai School of Medicine and The Rockefeller University, New York, NY

    • Medical Education:
    • Residency:
    • Fellowship(s):
    • Board Certification(s):
    • Affiliation(s):
    • Research Interests:
    • Primary Specialty:
    • Hospital:
    • Courses Taught:
      • Biomedical Sciences Program Track: Molecular Integrative Biosciences (MIB)
      • Biotechnology Program--Research Design (didactic, team-focused learning) 2014 - 2017
        Course Director since 2014

       Teaching

    • Current Projects:
    • Research Projects

      Functional Importance of the Purg/WRN complex
         We are investigating the little-studied protein Pur-gamma (Purg) and have found a novel interaction between Purg and WRN, a protein associated with the progeria disorder Werner Syndrome. We find that Purg and WRN interact in response to cellular stressors such as oncogenesis, neural precursor cellular reorganization and viral infection. PURG and WRN genes are located head to head and share a common promoter region. There are two isoforms of Purg, Purg-A and Purg-B, and we have recently developed monoclonal antibodies to distinguish these. Our current studies are designed to determine the functional importance of the Purg/WRN complex and the role of the two Purg isoforms in this complex. Purg is a member of the Pur family of proteins, and Pur family members have been shown to play important roles in brain development in mice. Other family members have been associated with the neurodegenerative disease, ALS. Our studies of Purg are, therefore, being conducted using glioblastoma cells as well as primary glial cells and neural precursors.

      Cellular, molecular analysis of MCM8 function
         We discovered the gene for MCM8, a new MCM family member that is not found in yeast, but which has evolved to adapt to the more complex nuclear structure of higher eukaryotes. We have also reported an additional variant of MCM8. Through genome-wide association studies, the MCM8 locus has been associated with the age at menarche and age at natural menopause. Whole-exome sequencing has revealed that MCM8 gene mutations result in primary gonadal failure and chromosome instability in the peripheral fibroblasts. In collaboration with members of The Jones Institute, our group is studying the function of MCM8 and MCM8 variants/isoforms in luteinized granulosa cells derived as byproducts from fertile oocyte donors. We are also studying the differential expression of MCM8 variants and their associated expression patterns in leukocytes from fertile egg donors and patients with several forms of primary ovarian failure.

      Co-investigator projects

      Adaptive Mechanism of DNA Replication-driven Recombination Alters JCV Control Region Sequences, EM Johnson, PI

      An Engineered, Synthetic, Pur-based Peptide for Treatment of Critical Aspects of ALS/FTD, EM Johnson, PI; EW Godfrey.

       

       

    • Bio: